Dexamethasone (DXM) ELISA BioAssay(TM) Kit

Catalog Number: USB-376896
Article Name: Dexamethasone (DXM) ELISA BioAssay(TM) Kit
Biozol Catalog Number: USB-376896
Supplier Catalog Number: 376896
Alternative Catalog Number: USB-376896-48,USB-376896-96
Manufacturer: US Biological
Category: Kits/Assays
Application: ELISA
Sample Type: Muscle, milk, feed Intended Use: This BioAssay(TM) kit allows for the in vitro quantitative determination of Dexamethasone concentrations in muscle, milk, feed. Sensitivity: 0.1ppb Range: 0.1-8.1ppb Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive inhibition enzyme immunoassay technique. The coupling antigen is pre-coated on the micro-well stripes. Then the Dexamethasone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Dexamethasone antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Dexamethasone in the standards or samples. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate, 96T Standard (96T), 6x1ml High Concentration Standard (100ppb) (96T), 1x1ml HRP-conjugate (96T), 1x11ml Antibody (96T), 1x5.5ml Substrate A (96T), 1x6ml Substrate B (96T), 1x6ml Stop Solution (96T), 1x6ml Redissolving Solution (2x) (96T), 1x50ml Wash Buffer (20X) (96T), 1x40ml Adhesive Strip (96T), 4x strips Instruction Manual (96T), 1x manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.