Penicillin G (Benzyl Penicillin) ELISA BioAssay(TM) Kit
Biozol Catalog Number:
USB-376908
Supplier Catalog Number:
376908
Alternative Catalog Number:
USB-376908-48,USB-376908-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
Sample Type: Tissue (chicken, porcine, liver). Intended Use: This BioAssay(TM) kit allows for the in vitro quantitative determination of Benzyl penicillin concentrations in tissue (chicken, porcine, liver). Sensitivity: 0.1ppb Range: 0.1-8.1ppb Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive enzyme immunoassay technique. The coupling antigen is pre-coated on the micro-well stripes. The Benzyl penicillin in the testing sample competes with the coupling antigens pre-coated on the micro-well stripes for the antibodies against Benzyl penicillin. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Benzyl penicillin in it. This value is compared to the standard curve and the content of the corresponding Benzyl penicillin is subsequently obtained. Kit Components: Assay plate, 96T Standard (96T), 6x1ml HRP-conjugate (96T), 1x12ml Antibody (96T), 1x7ml Substrate A (96T), 1x7ml Substrate B (96T), 1x7ml Stop Solution (96T), 1x7ml Wash Buffer (10X) (96T), 1x40ml Redissolving Solution (10x) (96T), 1x50ml Adhesive Strip (96T), 4x strips Instruction Manual (96T), 1x manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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