Phenylethanolamine A ELISA BioAssay(TM) Kit

Product Overview

• Article Name: Phenylethanolamine A ELISA BioAssay(TM) Kit
• Biozol Catalog Number: USB-376909
• Supplier Catalog Number: 376909
• Alternative Catalog Number: USB-376909-48,USB-376909-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Phenylethanolamine A, named as Phenethylamine, can accelerate animal growth, decrease fat deposition, increase the amount of lean meat as well as Ractopamine. Phenylethanolamine A is used as a new type of Clenbuterol and widely used on animal production. The general symptoms are nausea, dizziness, asthenia and fibrillation after eating high-Phenylethanolamine A-food, even chromosome aberration and cancer. Thus Phenylethanolamine A is listed as ''Prohibit material within animal and water on No.1519 from Ministry of Agriculture of the Peoples Republic of China. This BioAssay(TM) kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes. Sample Type: Urine Intended Use: This BioAssay(TM) kit allows for the in vitro quantitative determination of Phenylethanolamine A concentrations in urine. Sensitivity: 0.125ppb Range: 0.125-2ppb Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay(TM) kit has been pre-coated with Phenylethanola mine A antigen. Standards or samples are added to the appropriate microtiter plate wells with Phenylethanolamine A specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Phenylethanolamine A and Phenylethanolamine A in standards or samples with the Phenylethanolamine A special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Phenylethanolamine A in the standards or samples. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate, 96T/48T Standard (96T), 6x1ml Standard (48T), 6x0.5ml HRP-conjugate (96T), 1x7ml HRP-conjugate (48T), 1x3.5ml Antibody (96T), 1x7ml Antibody (48T), 1x1x3.5ml TMB Substrate (96T), 1x12ml TMB Substrate (48T), 1x6ml Stop Solution (96T), 1x10ml Stop Solution, (48T), 1x5ml Wash Buffer (10X) (96T), 1x50ml Wash Buffer (10X) (48T), 1x25ml Adhesive Strip (96T/48T), 4x strips Instruction Manual (96T/48T), 1x manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.