Zearalenone (ZEN) ELISA BioAssay(TM) Kit

Product Overview

• Article Name: Zearalenone (ZEN) ELISA BioAssay(TM) Kit
• Biozol Catalog Number: USB-376922
• Supplier Catalog Number: 376922
• Alternative Catalog Number: USB-376922-48,USB-376922-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

This BioAssay(TM) kit allows for the in vitro quantitative determination of Zearalenone concentrations in corn, wheat and grain corps, feed, raw materials. Sensitivity: 0.2ppb Range: 0.2-7.5ppb Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive enzyme immunoassay technique. The coupling antigen is pre-coated on the micro-well stripes. The Zearalenone in the testing sample competes with the coupling antigens pre-coated on the micro-well stripes for the antibodies against Zearalenone. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Zearalenone in it. This value is compared to the standard curve and the content of the corresponding Zearalenone is subsequently obtained. Kit Components: Assay plate, 96x wells Standard, 5x1ml HRP-conjugate, 1x6ml Antibody, 1x6ml Substrate A, 1x6ml Substrate B, 1x6ml Stop Solution, 1x6ml Sample Diluent (20X), 1x40ml Wash Buffer (10X), 1x40ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.