PRMT5, Chemiluminescent BioAssay(TM) Kit (Protein Arginine Methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1)

Product Overview

• Article Name: PRMT5, Chemiluminescent BioAssay(TM) Kit (Protein Arginine Methyltransferase 5, HRMT1L5, IBP72, JBP1, SKB1)
• Biozol Catalog Number: USB-377011
• Supplier Catalog Number: 377011
• Alternative Catalog Number: USB-377011-384
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. Intended Use: Suitable for studying enzyme kinetics and HTS applications. Test Principle: The PRMT5 Chemiluminescent BioAssay(TM) Kit is designed to measure PRMT5 activity for screening and profiling applications. The PRMT5 Chemiluminescent BioAssay(TM) Kit comes in a convenient format, with a 384-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified PRMT5 enzyme for 384 enzyme reactions. The key to the PRMT5 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3 residue of Histone H4. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt Kit Components: PRMT5/MEP50 human enzyme, 1x40ug 100uM S-adenosylmethionine, 1x250ul Primary antibody 4-3, 1x100ul Secondary HRP-labeled antibody 2, 2x10ul 4x PRMT5 assay buffer (add DTT before experiment), 2x3ml Blocking buffer, 2x50ml HRP chemiluminescent substrate A (transparent bottle), 2x6ml HRP chemiluminescent substrate B (brown bottle), 2x6ml 384-well plate precoated with histone substrate, 1x plate Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.