PRMT6, Homogeneous BioAssay(TM) Kit (HRMT1L6)

Product Overview

• Article Name: PRMT6, Homogeneous BioAssay(TM) Kit (HRMT1L6)
• Biozol Catalog Number: USB-377016
• Supplier Catalog Number: 377016
• Alternative Catalog Number: USB-377016-384
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3. Intended Use: Suitable for use in studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Test Principle: The PRMT6 Homogeneous BioAssay(TM) Kit is designed to measure PRMT6 activity for screening and profiling applications. PRMT6 is a histone methyltransferase that exhibits methylation activity toward histone H3-R2 and H4-R3. The PRMT6 Homogeneous BioAssay(TM) Kit comes in a convenient AlphaLISA format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT6 for 384 enzyme reactions. The key to the PRMT6 Homogeneous BioAssay(TM) Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT6 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen assays. Kit Components: PRMT6, 1x20ug 400uM S-adenosylmethionine, 1x250ul Primary antibody 4, 1x400ul Biotinylated histone H4 peptide substrate, 1x400ul 4x HMT assay Buffer 6, 1x3ml 4x Detection Buffer, 1x2ml Storage and Stability: Store powder at 4C liquid at -20C/-70C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.