CD99, also known as CD99 antigen isoform a, is a type 1 transmembrane glycoprotein and the founding member of the CD99 family of molecules. Cells known to express this protein include fibroblasts, neutrophils, T cells, CD34+ stem cells, monocytes and endothelial cells. Also, it plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Source: Recombinant protein corresponding to aa23-122 from human CD99, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~37.3kD (342aa), 40-57kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPDGGFDLS DALPDNENKK PTAIPKKPSA GDDFDLGDAV VDGENDDPRP PNPPKPMPNP NPNHPSSSGS FSDADLADGV SGGEGKGGSD GGGSHRKEGE EADLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
