CFB, Recombinant, Human, aa26-259, His-Tag (Complement Factor B, AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2)

Product Overview

• Article Name: CFB, Recombinant, Human, aa26-259, His-Tag (Complement Factor B, AHUS4, ARMD14, BF, BFD, CFAB, CFBD, FB, FBI12, GBG, H2-Bf, PBF2)
• Biozol Catalog Number: USB-377760
• Supplier Catalog Number: 377760
• Alternative Catalog Number: USB-377760-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CFB, also known as complement factor B, is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. Source: Recombinant protein corresponding to aa26-259 from human CFB, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~27.3kD (245aa), 28-40kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPEFTPWSL ARPQGSCSLE GVEIKGGSFR LLQEGQALEY VCPSGFYPYP VQTRTCRSTG SWSTLKTQDQ KTVRKAECRA IHCPRPHDFE NGEYWPRSPY YNVSDEISFH CYDGYTLRGS ANRTCQVNGR WSGQTAICDN GAGYCSNPGI PIGTRKVGSQ YRLEDSVTYH CSRGLTLRGS QRRTCQEGGS WSGTEPSCQD SFMYDTPQEV AEAFLSSLTE TIEGVDAEDG HGPGEQQKRH HHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 27.3
• NCBI: 001701
• UniProt: P00751
• Purity: ~95% (SDS-PAGE)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.