CNDP1, Recombinant, Human, aa27-507, His-Tag (Beta-Ala-His Dipeptidase, CNDP Dipeptidase 1, Carnosine Dipeptidase 1, Glutamate Carboxypeptidase-like Protein 2, CN1, CPGL2, HST2308)

Product Overview

• Article Name: CNDP1, Recombinant, Human, aa27-507, His-Tag (Beta-Ala-His Dipeptidase, CNDP Dipeptidase 1, Carnosine Dipeptidase 1, Glutamate Carboxypeptidase-like Protein 2, CN1, CPGL2, HST2308)
• Biozol Catalog Number: USB-377766
• Supplier Catalog Number: 377766
• Alternative Catalog Number: USB-377766-10,USB-377766-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells. Source: Recombinant protein corresponding to aa27-507 from human CNDP1, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~54.9kD (489aa), 50-70kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >3000pmol/min/ug, and as measured by the hydrolysis of carnosine/minute at pH 6.8 at 25C. Amino Acid Sequence: SPSPPPALLE KVFQYIDLHQ DEFVQTLKEW VAIESDSVQP VPRFRQELFR MMAVAADTLQ RLGARVASVD MGPQQLPDGQ SLPIPPVILA ELGSDPTKGT VCFYGHLDVQ PADRGDGWLT DPYVLTEVDG KLYGRGATDN KGPVLAWINA VSAFRALEQD LPVNIKFIIE GMEEAGSVAL EELVEKEKDR FFSGVDYIVI SDNLWISQRK PAITYGTRGN SYFMVEVKCR DQDFHSGTFG GILHEPMADL VALLGSLVDS SGHILVPGIY DEVVPLTEEE INTYKAIHLD LEEYRNSSRV EKFLFDTKEE ILMHLWRYPS LSIHGIEGAF DEPGTKTVIP GRVIGKFSIR LVPHMNVSAV EKQVTRHLED VFSKRNSSNK MVVSMTLGLH PWIANIDDTQ YLAAKRAIRT VFGTEPDMIR DGSTIPIAKM FQEIVHKSVV LIPLGAVDDG EHSQNEKINR WNYIEGTKLF AAFFLEMAQL HLEHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 54.9
• NCBI: 116038
• UniProt: Q96KN2
• Purity: 95% (SDS-PAGE)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.