Eif4e, Recombinant, Mouse, aa1-217, His-Tag (Eukaryotic Translation Initiation Factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e)

Product Overview

• Article Name: Eif4e, Recombinant, Mouse, aa1-217, His-Tag (Eukaryotic Translation Initiation Factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e)
• Biozol Catalog Number: USB-377794
• Supplier Catalog Number: 377794
• Alternative Catalog Number: USB-377794-10,USB-377794-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Source: Recombinant protein corresponding to aa1-217 from mouse Eif4e, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~27.6kD (241aa), confirmed by MALDI-TOF AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMATVEP ETTPTTNPPP AEEEKTESNQ EVANPEHYIK HPLQNRWALW FFKNDKSKTW QANLRLISKF DTVEDFWALY NHIQLSSNLM PGCDYSLFKD GIEPMWEDEK NKRGGRWLIT LNKQQRRSDL DRFWLETLLC LIGESFDDYS DDVCGAVVNV RAKGDKIAIW TTECENRDAV THIGRVYKER LGLPPKIVIG YQSHADTATK SGSTTKNRFV V Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 27.6
• NCBI: 031943
• UniProt: P63073
• Purity: ~95% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1mM PMSF, 0.1M sodium chloride, 10% glycerol.