GUSB, Recombinant, Human, aa23-651, His-Tag (Beta-glucuronidase Isoform 1, BG, MPS7)

Product Overview

• Article Name: GUSB, Recombinant, Human, aa23-651, His-Tag (Beta-glucuronidase Isoform 1, BG, MPS7)
• Biozol Catalog Number: USB-377829
• Supplier Catalog Number: 377829
• Alternative Catalog Number: USB-377829-5,USB-377829-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Source: Recombinant protein corresponding to aa23-651 from human GUSB, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~73.4kD (635aa), 70-100kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >1600pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferone to 4- Methylum-belliferyl-beta-D-glucosiduronic acid/minute at 37C and pH 6.0. Amino Acid Sequence: LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 73.4
• NCBI: 000172
• UniProt: P08236
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.