IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Source: Recombinant protein corresponding to aa34-356 from mouse IMPAD1, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~36.2kD (332aa), 28-40kD (SDS-PAGE under non-reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Biological Activity: Specific activity is >5000pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate/minute at pH 7.5 at 25C. Amino Acid Sequence: ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.