Etanercept (Enbrel) BioAssay(TM) ELISA Kit

Product Overview

• Article Name: Etanercept (Enbrel) BioAssay(TM) ELISA Kit
• Biozol Catalog Number: USB-384861
• Supplier Catalog Number: 384861
• Alternative Catalog Number: USB-384861-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Etanercept (Enbrel) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF, a soluble inflammatory cytokine). Etanercept is a fusion protein of the extracellular ligand binding portion of TNFRSF1B and the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF. The Etanercept ELISA kit is designed to measure free Etanercept with high specificity and sensitivity in biological matrices. Sample Type: Serum and plasma Detection Range: 78-2500ng/ml Detection Limit: 1.5ng/ml Recovery: 250ng/ml of Etanercept was spiked in 10 lots of human serum. Recovery ranges are from 91-112% with an average recovery of 110%. Hook Effect: No hook effect was observed up to 40000ng/ml of Etanercept. Precision: Intra-assay CV: <10% Inter-assay CV: <10% Specificity: sTNFa, TNFb, sTNFRI, sTNFRII prepared at 250ng/ml were assayed and exhibited no crossreactivity or interference. Test Principle: This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured. Kit Components: 384861A: Microtiter Strips, 1x96 wells 384861B: Calibrator Diluent, 1x1.8ml 384861C: Calibrator (500ug/ml), 1x12ul 384861D: Wash Buffer, 10X, 1x50ml 384861E: Assay Buffer, 1x50ml 384861F: Detection Reagent, 1000X, 1x17ul 384861G: TMB, 1x12ml 384861H: Stop Solution, 1x12ml Storage and Stability: Store kit components at -20C unless specified otherwise. Unopened kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge small tubes on pulse setting prior to opening.