CENP-A (CENP A, Cenpa, Centromere Auto Antigen A, Histone H3 Like Centromeric Protein A), IgG1, Clone: [5A7-2E11], Mouse, Monoclonal
Catalog Number:
USB-384873
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| Article Name: | CENP-A (CENP A, Cenpa, Centromere Auto Antigen A, Histone H3 Like Centromeric Protein A), IgG1, Clone: [5A7-2E11], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-384873 |
| Supplier Catalog Number: | 384873 |
| Alternative Catalog Number: | USB-384873-100 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, WB |
| Immunogen: | Synthetic peptide corresponding to a portion of human CENP-A. |
| A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome. Applications: Suitable for use in Western Blot, Immunocytochemistry/Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry/Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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