8-Hydroxydeoxyguanosine (8-OHdG) BioAssay(TM) ELISA Kit

Product Overview

• Article Name: 8-Hydroxydeoxyguanosine (8-OHdG) BioAssay(TM) ELISA Kit
• Biozol Catalog Number: USB-385070
• Supplier Catalog Number: 385070
• Alternative Catalog Number: USB-385070-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21pg/ml as determined by LC-MS (10-11). Sample Type: Cell lysates, Plasma, Sample matrices, Urine Intended Use: This ELISA BioAssay(TM) kit is used for 8-OHdG detection in samples. Sensitivity: 0.59ng/mL Range: 0.94-60ng/mL Specificity: Species Independent Test Principle: This ELISA BioAssay(TM) Kit is a competitive assay that can be used for the quantification of 8-OHdG in urine, cell culture, plasma, and other sample matrices. The ELISA utilizes an 8-hydroxy-2-deoxy Guanosine-coated plate and an HRP-conjugated antibody for detection which allows for an assay range of 0.94-60ng/mL, with a sensitivity of 0.59ng/mL. The other highlights of this BioAssay(TM) kit are a quick incubation time of 60 minutes, stable reagents, and an easy to use protocol. It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This should be kept in mind when analyzing and interpreting experimental results. Kit Components: BSA Coated Plate, 1x96wells Standard, 1x100uL HRP Conjugated Monoclonal Antibody, 1x75uL Sample and Standard Diluent, 1x50mL Antibody Diluent, 1x13mL Wash Buffer Concentrate, 1x50mL TMB Substrate, 1x13mL Stop Solution, 1x13mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.