Platelet Activating Factor (PAF) BioAssay(TM) ELISA Kit (General)

Product Overview

• Article Name: Platelet Activating Factor (PAF) BioAssay(TM) ELISA Kit (General)
• Biozol Catalog Number: USB-396541
• Supplier Catalog Number: 396541
• Alternative Catalog Number: USB-396541-48,USB-396541-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Platelet Activating Factor (PAF) BioAssay(TM) ELISA Kit is a quantitative competitive assay for the detection of PAF in serum, plasma, tissue homogenates and other biological fluids. Detection Range: 1.56-100ng/ml Sensitivity: <0.938ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with PAF. PAF in the standard or sample competes with a fixed amount of plate-coated PAF for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of PAF in the samples is determined by comparing the absorbance of the samples to the standard curve. Kit Components: *396541A: Microtiter Strips, 8x12 wells. *396541B: Standard, 2 x1vial 396541C: Sample/Standard Dilution Buffer, 1x20ml 396541D: Antibody-Biotin Concentrate, 1x60ul 396541E: Antibody Dilution Buffer, 1x10ml 396541F: Streptavidin-HRP Conjugate (SABC), 1x120ul 396541G: SABC Dilution Buffer, 1x10ml 396541H: TMB Substrate, 1x10ml 396541J: Stop Solution, 1x10ml 396541K: Wash Buffer, 25X, 1x30ml Storage and Stability: Store unopened *396541A at 4C, store at -20C once opened. Store unopened *396541B at 4C, once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 50ul standard or sample to each well. 3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.