Alpha-1-B glycoprotein is a 54.3kD protein in humans that is encoded by the A1BG gene. The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human A1BG/alpha 1B-Glycoprotein. Sensitivity: <10pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant Human A1BG/alpha 1B-Glycoprotein. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human A1BG/alpha 1B-Glycoprotein BioAssay(TM) ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for A1BG/alpha 1B-Glycoprotein has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A22-S495) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for A1BG/alpha 1B-Glycoprotein is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human A1BG/alpha 1B-Glycoprotein amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human A1BG antibody, 1x plate Lyophilized recombinant Human A1BG standard, 2x10ng/tube Biotinylated anti-Human A1BG antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
