BMP-2 (Bone Morphogenetic Protein 2) BioAssay(TM) ELISA Kit (Porcine)
Biozol Catalog Number:
USB-396574
Supplier Catalog Number:
396574
Alternative Catalog Number:
USB-396574-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF- beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant Porcine BMP-2, constituting dimer by two chains of 114aa with the molecular mass of 26kD. Sample Type: Cell culture supernates, serum, bone tissue, tissue. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Porcine BMP-2. Sensitivity: <2pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant Porcine BMP-2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine BMP-2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine BMP-2 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine BMP-2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine BMP-2 antibody, 1x plate Lyophilized recombinant Porcine BMP-2 standard, 2x10ng/tube Biotinylated anti-Porcine antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
