BMP-4 (Bone Morphogenetic Protein 4) BioAssay(TM) ELISA Kit (Bovine)
Biozol Catalog Number:
USB-396576
Supplier Catalog Number:
396576
Alternative Catalog Number:
USB-396576-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In Bovine embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26kD. Sample Type: Bone tissue and cell culture supernates. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Bovine BMP-4. Sensitivity: <4pg/ml Range: 62.5pg/ml-4000pg/ml Specificity: Recognizes natural and recombinant Bovine BMP-4. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Bovine BMP-4 ELISA BioAssay(TM) Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Bovine BMP-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Bovine BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine BMP-4 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Bovine BMP-4 antibody, 1x plate Lyophilized recombinant Bovine BMP-4 standard, 2x10ng/tube Biotinylated anti-Bovine BMP-4 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
