Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kD, consisting of 247aa. It is known to promote neuronal survival and differentiation. BDNF shares substantial aa sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult rabbit brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding rabbit BDNF to chromosome 11, band p13. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rabbit BDNF. Sensitivity: <15pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant rabbit BDNF. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rabbit BDNF BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit BDNF has been precoated onto 96-well plates. Standards (Expression system for standard: sf21, Immunogen sequence: H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit BDNF amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rabbit BDNF antibody, 1x plate Lyophilized recombinant Rabbit BDNF standard, 2x10ng/tube Biotinylated anti-Rabbit BDNF antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
