Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kD, consisting of 247aa. It is known to promote neuronal survival and differentiation. BDNF shares substantial aa sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult Bovine brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding Bovine BDNF to chromosome 11, band p13. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA). Intended Use: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Bovine BDNF. Sensitivity: <15pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant bovine BDNF. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Bovine BDNF BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Bovine BDNF has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: H129-R247) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Bovine BDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine BDNF amount of sample captured in plate. Kit Components: 396594A: Microtiter Strips, 1x96 wells 396594B: BDNF Standard, 2x1 vial 396594C: Anti-canine BDNF (Biotin), 1x130ul 143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul 143697: Sample Diluent Buffer, 1x30ml 143698: Antibody Diluent Buffer, 1x12ml 143699: ABC Diluent Buffer, 1x12ml 143700: TMB Color Developing Agent, 1x10ml 143701: TMB Stop Solution, 2N H2SO4, 1x10ml Storage and Stability: Store *396594B powder at 4C. Once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
