Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Equine CXCL14. Sensitivity: <10pg/ml Range: 62.5pg/ml-4000pg/ml Specificity: Recognizes natural and recombinant Equine CXCL14. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Equine CXCL14 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Equine CXCL14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Equine CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine CXCL14 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Equine CXCL14 antibody, 1x plate Lyophilized recombinant Equine CXCL14 standard, 2x10ng/tube Biotinylated anti-Equine CXCL14 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
