Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminal epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminal may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Porcine Epiregulin. Sensitivity: <10pg/ml Range: 156pg/ml-10,000pg/ml Specificity: Recognizes natural and recombinant Porcine Epiregulin. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine Epiregulin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine Epiregulin has been precoated onto 96-well plates. Standards(E.coli, V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine Epiregulin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine EREG antibody, 1x plate Lyophilized recombinant Porcine EREG standard, 2x10ng/tube Biotinylated anti-Porcine EREG antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
