Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The Rabbit FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208aa. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia. As a result of glycosylation, the molecular mass is 25-27kD. Sample Type: Cell culture supernates, serum, plasma (heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Rabbit FGF9. Sensitivity: <15pg/ml Range: 62.5pg/ml-4000pg/ml Specificity: Recognizes natural and recombinant Rabbit FGF9. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Rabbit FGF9 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Rabbit FGF9 has been precoated onto 96-well plates. Standards(Expression system for standard: sf21, Immunogen sequence: M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Rabbit FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rabbit FGF9 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Rabbit FGF9 antibody, 1x plate Lyophilized recombinant Rabbit FGF9 standard, 2x10ng/tube Biotinylated anti-Rabbit FGF9 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
