Folate receptor beta is a protein that in humans is encoded by the FOLR2 gene. The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. Sample Type: Cell culture supernates, serum. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human FOLR2/Beta Fr. Sensitivity: <1pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant Human FOLR2/Beta Fr. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human FOLR2/Beta Fr BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for FOLR2/Beta Fr has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: T17-N230) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FOLR2/Beta Fr is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FOLR2/Beta Fr amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human FOLR2 antibody, 1x plate Lyophilized recombinant Human FOLR2 standard, 2x10ng/tube Biotinylated anti-Human FOLR2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
