TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several neurotrophins, which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A tyrosine kinase is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or substrates. Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. Sample Type: Cell lysates, cell culture supernates. Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human TrkB. Sensitivity: <10pg/ml Range: 156pg/ml-10,000pg/ml Specificity: Recognizes natural and recombinant Human TrkB. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human TrkB BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for TrkB has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: C32-H430) and test samples are added to the wells, a biotinylated detection specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TrkB amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human NTRK2 antibody, 1x plate Lyophilized recombinant Human NTRK2 standard, 2x10ng/tube Biotinylated anti-Human NTRK2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
