ART4, also known as ecto-ADP-ribosyltransferase 4, is a glycosylphosphatidylinositol (GPI)-linked membrane protein carrying Dombrock blood group antigens. It is developmentally regulated during erythroid differentiation and occurs at highest levels in the fetal liver. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Recombinant human ART4, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa47-285 of human ART4, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~28.8kD (248aa), ~28-40kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPSEVAIKI DFDFAPGSFD DQYQGCSKQV MEKLTQGDYF TKDIEAQKNY FRMWQKAHLA WLNQGKVLPQ NMTTTHAVAI LFYTLNSNVH SDFTRAMASV ARTPQQYERS FHFKYLHYYL TSAIQLLRKD SIMENGTLCY EVHYRTKDVH FNAYTGATIR FGQFLSTSLL KEEAQEFGNQ TLFTIFTCLG APVQYFSLKK EVLIPPYELF KVINMSYHPR GDWLQLRSTG NLSTYNCQLL KAHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
