Egf, also known as Pro-epidermal growth factor isoform1, is a 8.6kD globular protein containing 77aa residues, including 3 intra-molecular disulfide bonds. Egf is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, Egf has been shown to inhibit gastric secretion, and to be involved in wound healing. Egf signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse Egf, fused to His-Tag at N-terminal, expressed in E. coli and purified by conventional column chromatography. Source: Recombinant protein corresponding to aa977-1029 of mouse Egf, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~8.6kD (77aa), Confirmed by MALDI-TOF Endotoxin: <0.2EU/1ug (determined by LAL method) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA Biological Activity: Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 for this effect less or equal to 1ng/ml. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
