HLA-G, Recombinant, Human, aa25-308, His-Tag (HLA Class I Histocompatibility Antigen, Alpha Chain G, HLA Class I Histocompatibility Antigen, Alpha Chain G)
Biozol Catalog Number:
USB-396877
Supplier Catalog Number:
396877
Alternative Catalog Number:
USB-396877-20,USB-396877-100
Manufacturer:
US Biological
Category:
Molekularbiologie
HLA-G, also known as HLA class I histocompatibility antigen, alpha chain G, belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. HLA-G is a non-classical class-I HLA molecule associated with anti-inflammatory and immuno- modulatory properties which interacts with inhibitory receptors (ILT2/ILT4/KIR2DL4) present on various immune cells. It inhibits proliferation of B cells, T cells and natural killer cells, and also induces regulatory T cells. Recombinant human HLA-G, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa25-308 of human CLMP, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~35.3kD (309aa), Confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGSHSM RYFSAAVSRP GRGEPRFIAM GYVDDTQFVR FDSDSACPRM EPRAPWVEQE GPEYWEEETR NTKAHAQTDR MNLQTLRGYY NQSEASSHTL QWMIGCDLGS DGRLLRGYEQ YAYDGKDYLA LNEDLRSWTA ADTAAQISKR KCEAANVAEQ RRAYLEGTCV EWLHRYLENG KEMLQRADPP KTHVTHHPVF DYEATLRCWA LGFYPAEIIL TWQRDGRDQT QDVELVETRP AGDGTFQKWA AVVVPSGEEQ RYTCHVQHEG LPEPLMLRWK QSSLPTIPI Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
