NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa396-678 of human NFATC2, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~33.1kD (290aa), ~28-40kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: PLEWPLSSQS GSYELRIEVQ PKPHHRAHYE TEGSRGAVKA PTGGHPVVQL HGYMENKPLG LQIFIGTADE RILKPHAFYQ VHRITGKTVT TTSYEKIVGN TKVLEIPLEP KNNMRATIDC AGILKLRNAD IELRKGETDI GRKNTRVRLV FRVHIPESSG RIVSLQTASN PIECSQRSAH ELPMVERQDT DSCLVYGGQQ MILTGQNFTS ESKVVFTEKT TDGQQIWEME ATVDKDKSQP NMLFVEIPEY RNKHIRTPVK VNFYVINGKR KRSQPQHFTY HPVHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
