RPS6KB1, Recombinant, Human, aa1-525, His-Tag (Ribosomal Protein S6 Kinase Beta-1 Isoform A, RPS6KB1, p70 S6KA, p70(S6K)-Alpha, p70-Alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A)

Product Overview

• Article Name: RPS6KB1, Recombinant, Human, aa1-525, His-Tag (Ribosomal Protein S6 Kinase Beta-1 Isoform A, RPS6KB1, p70 S6KA, p70(S6K)-Alpha, p70-Alpha, p70-S6K, PS6K, S6K, S6K-beta-1, S6K1, STK14A)
• Biozol Catalog Number: USB-396923
• Supplier Catalog Number: 396923
• Alternative Catalog Number: USB-396923-10,USB-396923-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

RPS6KB1, also known as ribosomal protein S6 kinase beta-1 isoform a, is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. Phosphorylation of S6 induces protein synthesis at the ribosome. The phosphorylation of p70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of p70S6K plays a more positive role in the increase of autophagy. Recombinant human RPS6KB1, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa1-525 of human RPS6KB1, fused to His-Tag at C-terminal, expressed in insect cell. Accession Number: NP_003152 Molecular Weight: ~60.2kD (533aa) Amino Acid Sequence: MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNLLEHHH HHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 60.2
• NCBI: 003152
• UniProt: P23443
• Purity: 85% (SDS-PAGE). Endotoxin: 1EU/ug (LAL).
• Form: Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 50mM sodium chloride, 2mM DTT, 0.1mM PMSF, 40% glycerol.