Hygromycin-B 4-O-kinase (APH(4) Hygromycin B phosphotransferase Hygromycin-B kinase) Recombinant, E. Coli, aa1-341, His-tag (hph)

Product Overview

• Article Name: Hygromycin-B 4-O-kinase (APH(4) Hygromycin B phosphotransferase Hygromycin-B kinase) Recombinant, E. Coli, aa1-341, His-tag (hph)
• Biozol Catalog Number: USB-405952
• Supplier Catalog Number: 405952
• Alternative Catalog Number: USB-405952-20,USB-405952-200
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin. Source: Recombinant protein corresponding to aa1-341 from E. Coli Hygromycin-B 4-O-kinase, fused to His-tag at N-terminal, expressed in E. coli. Molecular Weight: ~42.0kD Amino Acid Sequence: MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 42
• UniProt: P00557
• Purity: ~85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.