Transcriptional Regulatory Protein PhoP, Recombinant, E. coli, aa1-223, His-tag, Myc-tag (phoP)

Product Overview

• Article Name: Transcriptional Regulatory Protein PhoP, Recombinant, E. coli, aa1-223, His-tag, Myc-tag (phoP)
• Biozol Catalog Number: USB-406054
• Supplier Catalog Number: 406054
• Alternative Catalog Number: USB-406054-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes. Full length recombinant protein corresponding to aa1-223 from Escherichia Coli Transcriptional regulatory protein PhoP, fused to His-tag at N-terminal and fused to Myc-tag at C-terminal, expressed in Mammalian cell. Molecular Weight: ~29.5kD Amino Acid Sequence: MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHIPDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFELR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 29.5
• UniProt: P23836
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.