The antigen preparation contains a high concentration of virus and viral components as well as some cellular material in MEM. Source: Adenovirus, strain Adenoid 6, from MRC-5. Applications: Suitable for use in both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Adenovirus antigen is inactivated using gamma radiation. This procedure is effective primarily by damaging viral genetic material. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Optimally infected cells are disrupted by sonication and then subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow ultrafiltration with a 30,000 molecular weight cut-off.
Form:
Supplied as a liquid in MEM.
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