Gram-negative pleomorphic cells, spherical to ovoid shaped, single tripled-layered membrane, but lacking true cell wall. Causes lower respiratory infections, less often a pharyngitis progressing to bronchitis or pneumoniae, gradual onset with headache, malaise, paroxysmal cough, substernal pain, leucocytosis in some cases. Duration varies from a few days to a month or more. Low fatality. Source: Mycoplasma pneumoniae, strain FH, from Broth base medium Applications: Suitable for use in both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Detergent inactivation by lysis. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Mycoplasma organisms are harvested by centrifugation, washed in PBS and extracted using non-ionic detergent-containing buffer.
Form:
Supplied as a liquid in phosphate, non-ionic detergent, sugar based stabilizer.
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