The predominant form of the antigen is whole virions suspended in NTE with sucrose. The preparation is free of detergent. Residual non-viral protein may come from host tissue and culture medium. Source: Rubella BR2S, strain HPV-77, from Vero cells. Applications: Suitable for use in a variety of immunoassay formats including micro well ELISA, latex agglutination and IgM detection assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: UV irradiation and confirmed by double passage assay on Vero cells. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Vero cells infected with Rubella release virus particles into the culture supernatant. Supernatant is harvested and clarified. The clarified material is then concentrated and purified by sucrose gradient centrifugation.
Form:
Supplied as a liquid in NTE (sodium chloride, TRIS, EDTA).
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