The APEX1 BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor APEX1 protein expression profile in cells. It can be used for measuring the relative amounts of APEX1 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on APEX1. Sensitivity: >5000cells/well Kit Components: 471716A: Microtiter Strips, 1x96 wells 471716B: TBS, 10X, 1x24ml 471716C: Quenching Buffer, 1x24ml 471716D: Blocking Buffer, 1x50ml 471716E: Wash Buffer, 15X, 1x50ml *471716F: APEX1 Pab, 100X, 1x60ul *471716G: GAPDH Mab, 100X, 1x60ul 471716H: IgG Pab (HRP), 1x6ml 471716J: IgG Mab (HRP), 1x6ml 471716K: Primary Antibody Diluent, 1x12ml 471716L: Substrate, 1x12ml 471716M: Stop Solution (2N Sulfuric Acid), 1x12ml 471716N: Crystal Violet Solution, 1x6ml *471716P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *471716F and *471716G are stable for 2 weeks at 4C.. *471716P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
