The CKMT2 BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor target CKMT2 protein and expression profile in cells. It can be used for measuring the relative amounts of CKMT2 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on CKMT2. Sensitivity: >5000cells/well Kit Components: 471887A: Microtiter Strips, 1x96 wells 471887B: TBS, 10X, 1x24ml 471887C: Quenching Buffer, 1x24ml 471887D: Blocking Buffer, 1x50ml 471887E: Wash Buffer, 15X, 1x50ml *471887F: CKMT2 Pab, 100X, 1x60ul *471887G: GAPDH Mab, 100X, 1x60ul 471887H: IgG Pab (HRP), 1x6ml 471887J: IgG Mab (HRP), 1x6ml 471887K: Primary Antibody Diluent, 1x12ml 471887L: Substrate, 1x12ml 471887M: Stop Solution (2N Sulfuric Acid), 1x12ml 471887N: Crystal Violet Solution, 1x6ml *471887P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *471887F and *471887G are stable for 2 weeks at 4C. *473193P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
