The HSP27 Colorimetric Cell-Based ELISA Kit can be used for measuring the amount of HSP27 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on HSP27. Sensitivity: >5000cells/well Kit Components: 472291A: Microtiter Strips, 1x96 wells 472291B: TBS, 10X, 1x24ml 472291C: Quenching Buffer, 1x25ml 472291D: Blocking Buffer, 1x50ml 472291E: Wash Buffer, 15X, 1x50ml *472291F: HSP Pab, 100X, 1x60ul *472291G: GAPDH Mab, 100X, 1x60ul 472291H: IgG Pab (HRP), 1x6ml 472291J: IgG Mab (HRP), 1x6ml 472291K: Primary Antibody Diluent, 1x12ml 472291L: Substrate, 1x12ml 472291M: Stop Solution (2N Sulfuric Acid), 1x12ml 472291N: Crystal Violet Solution, 1x6ml *472291P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472291F and *472291G are stable for 2 weeks at 4C.. *472291P should be stored at RT or wrmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
