The IRF4 Colorimetric Cell-Based BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IRF4 protein exprsssion profile in cells. It can be used for measuring the relative amounts of IRF4 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on IRF4. Sensitivity: >5000cells/well Kit Components: 472435A: Microtiter Strips, 1x96 wells 472435B: TBS, 10X, 1x24ml 472435C: Quenching Buffer, 1x24ml 472435D: Blocking Buffer, 1x50ml 472435E: Wash Buffer, 15X, 1x50ml *472435F: IRF4 Pab, 100X, 1x60ul *472435G: GAPDH Mab, 100X, 1x60ul 472435H: IgG Pab (HRP), 1x6ml 472435J: IgG Mab (HRP), 1x6ml 472435K: Primary Antibody Diluent, 1x12ml 472435L: Substrate, 1x12ml 472435M: Stop Solution (2N Sulfuric Acid), 1x12ml 472435N: Crystal Violet Solution, 1x6ml *472435P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472435F and *472435G are stable for 2 weeks at 4C. *472435P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
