Sample Type: Cell line of choice Intended Use: The Phospho-Smad1 (Ser465) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-Smad1(Ser465) in plated and fixed cells. Sensitivity: >5000cells/well Assay Time: 4.5 hours Reactivity: Human:S465, Mouse:S465, Rat:S468 Detection Method: Colorimetric 450 nm Format: Cell-Based ELISA Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.