Autophagy BioAssay(TM) Kit, Red

Product Overview

• Article Name: Autophagy BioAssay(TM) Kit, Red
• Biozol Catalog Number: USB-474002
• Supplier Catalog Number: 474002
• Alternative Catalog Number: USB-474002-50,USB-474002-200
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: FC

Product Description

Sample Type: Cell culture Excitation/Emission: 590nm/620nm Method of Analysis: Flow Cytometry Intended Use: Autophagy BioAssay(TM), Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometry. Test Principle: Autophagy BioAssay(TM), Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometry. Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications in cancer, infection, and degenerative diseases. Autophagy is a three stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs. Kit Components: 50 tests: Autophagy Probe, Red, 1 vial 10X Cellular Assay Buffer, 1x15mL Fixative, 1x6mL 200 tests: Autophagy Probe, Red, 4 vials 10X Cellular Assay Buffer, 1x60mL Fixative, 1x6mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.