Basic Cytotoxicity Test BioAssay(TM) Kit

Product Overview

• Article Name: Basic Cytotoxicity Test BioAssay(TM) Kit
• Biozol Catalog Number: USB-474004
• Supplier Catalog Number: 474004
• Alternative Catalog Number: USB-474004-125,USB-474004-250
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: FC

Product Description

Sample Type: Cell culture Reagent Name: 7-AAD, CFSE Target: Cell-mediated cytotoxicity Excitation/Emission: 7-AAD-546nm/647nm, CFSE-492nm/520nm Method of Analysis: Flow cytometry Intended Use: Basic Cytotoxicity Test Assay is a single-tube, dual-color assay for determining cytotoxicity by flow cytometry. The BioAssay(TM) employs a green fluorescent cellular stain, CFSE, to label target cells and the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity BioAssay(TM) samples. Analyze your results using a flow cytometer. Test Principle: Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Basic Cytotoxicity Assay allows you to assess cytolytic activity in cell culture. The Basic Cytotoxicity BioAssay(TM) includes two fluorescent reagents: CFSE and 7-AAD. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. The target cell and effector cell populations can then be easily distinguished, and flow cytometry easily identifies live and necrotic cells. Kit Components: 125 tests: CFSE target cell stain, 1 vial 7-AAD vital stain, 1 vial 10X Assay Buffer, 1x30mL 250 tests: CFSE target cell stain, 1 vial 7-AAD vital stain, 2 vials 10X Assay Buffer, 1x60mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.