FAM FLICA Caspase 3/7 BioAssay(TM) Kit

Product Overview

• Article Name: FAM FLICA Caspase 3/7 BioAssay(TM) Kit
• Biozol Catalog Number: USB-474006
• Supplier Catalog Number: 474006
• Alternative Catalog Number: USB-474006-25,USB-474006-100
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Sample Type: Cell culture Reagent Name: FAM-DEVD-FMK Target: Caspase-3/7 Excitation/Emission: 488nm/530nm Method of Analysis: Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader Intended Use: Detect caspase-3/7 activity with the FLICA Caspase-3/7 BioAssay(TM) Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry. Test Principle: Caspases play important roles in apoptosis and inflammation. The FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-3/7 assay probe allows researchers to assess caspase 3 and 7 activation. The FLICA reagent FAM-DEVD-FMK enters each cell and irreversibly binds to active caspases 3 and 7. Because the FAM-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound FAM-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively. Kit Components: 25 tests: FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial 10X Apoptosis Wash Buffer, 1x15ml Fixative, 1x6ml Propidium Iodide, 1x1ml Hoechst 33342, 1x1ml 100 tests: FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials 10X Apoptosis Wash Buffer, 1x60ml Fixative, 1x6ml Propidium Iodide, 1x1ml Hoechst 33342, 1x1ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.