Magic Red(TM) Caspase 3 & 7 BioAssay(TM) Kit

Product Overview

• Article Name: Magic Red(TM) Caspase 3 & 7 BioAssay(TM) Kit
• Biozol Catalog Number: USB-474029
• Supplier Catalog Number: 474029
• Alternative Catalog Number: USB-474029-25,USB-474029-100
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Magic Red(TM) Caspase 3 & 7 BioAssay(TM) Kit detects caspase 3 and 7 activity in real time. The Magic Red reagent fluoresces upon cleavage by active caspase 3 and 7 enzymes. Analyze the fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or flow cytometry. Test Principle: Caspases play important roles in apoptosis and inflammation. Detection of DEVDase enzyme activity (caspase 3 activity) is a reliable method for assessing apoptotic activity in experimental cell populations. The presence of the active form of caspase 3 & 7 in apoptotic cells causes the hydrolysis of the two DEVD target sequences from the Magic Red cresyl violet fluorophore, converting it to the fluorescent form. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. Magic Red excites at 590nm and emits at 628nm and can be analyzed using fluorescence microscopy, a fluorescent plate reader, or flow cytometry. Samples may also be analyzed with Acridine Orange dye or Hoechst stain to visualize lysosomal organelle structure or detect changes in nuclear morphology respectively. Excitation/Emission: 590nm/628nm Kit Components: *474029A: Magic Red Caspase-3/7 Substrate MR-(DEVD)2 Reagent, 1x1vial 474029B: Hoechst 33342 Stain (200ug/ml), 1x1ml 474029C: Acridine Orange Stain (1mM, 266ug/ml), 1x500ul Storage and Stability: Store *474029A powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.