Magic Red(TM) Cathepsin B BioAssay(TM) Kit

Product Overview

• Article Name: Magic Red(TM) Cathepsin B BioAssay(TM) Kit
• Biozol Catalog Number: USB-474030
• Supplier Catalog Number: 474030
• Alternative Catalog Number: USB-474030-25,USB-474030-100
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Sample Type: Cell culture Reagent Name: MR-RR2 Target: Cathepsin-B Excitation/Emission: 592nm/628nm Method of Analysis: Fluorescence Plate Reader, Fluorescence Microscope Intended Use: Quantitate and monitor intracellular cathepsin-B activity over time in vitro. The Magic Red substrate in this BioAssay(TM) fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescence plate reader. Test Principle: Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions. Magic Red BioAssay(TM) kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This BioAssay(TM) kit detects cathepsin-B activity. To use Magic Red, add the substrate directly to the cell culture media, incubate, and analyze. Because it is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles - no lysis or permeabilization steps are required. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophore will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or with a fluorescence plate reader. Hoechst 33342 and Acridine Orange are included in the BioAssay(TM) kit to detect nuclear morphology and lysosomal organelle structure, respectively. Kit Components: 25 tests: Magic Red Substrate (MR-RR2), 1 25 Test Vial Hoechst 33342, 1x1mL Acridine Orange, 1x0.5mL 100 tests: Magic Red Substrate (MR-RR2), 1 100 Test Vial Hoechst 33342, 1x1mL Acridine Orange, 1x0.5mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.