Magic Red(TM) Cathepsin L BioAssay(TM) Kit

Product Overview

• Article Name: Magic Red(TM) Cathepsin L BioAssay(TM) Kit
• Biozol Catalog Number: USB-474032
• Supplier Catalog Number: 474032
• Alternative Catalog Number: USB-474032-25,USB-474032-100
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Sample Type: Cell culture Reagent Name: MR-FR2 Target: Cathepsin L Excitation/Emission: 592nm/628nm Method of Analysis: Fluorescent Plate Reader, Fluorescence Microscope Intended Use: Quantitate and monitor intracellular cathepsin L activity over time in vitro. The Magic Red reagent in this BioAssay(TM) fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescent plate reader. Test Principle: Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions. Magic Red BioAssay(TM) kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This Magic Red kit detects cathepsin L The Magic Red reagent MR-FR2 enters each cell in a non-fluorescent state. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophone will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or fluorescent plate reader. Samples may also be analyzed with Acridine Orange or Hoechst stain to detect lysosomal organelle structure or nuclear morphology respectively. Kit Components: 25 tests: Magic Red Reagent (MR-FR2), 1 25-test vial Hoechst 33342 Stain, 1x1mL Acridine Orange Stain, 1x0.5mL 100 tests: Magic Red Reagent (MR-FR2), 1 100-test vial Hoechst 33342 Stain, 1x1mL Acridine Orange Stain, 1x0.5mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.