Necrosis vs Apoptosis BioAssay(TM) Kit

Product Overview

• Article Name: Necrosis vs Apoptosis BioAssay(TM) Kit
• Biozol Catalog Number: USB-474036
• Supplier Catalog Number: 474036
• Alternative Catalog Number: USB-474036-50,USB-474036-100
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: FC

Product Description

Sample Type: Cell culture Target: FAM-VAD-FMK, 7-AAD Excitation/Emission: FAM-FLICA - 492nm/529nm 7-AAD - 546nm/647nm Method of Analysis: Flow Cytometry, Fluorescence Microscope Intended Use: Necrosis vs Apoptosis BioAssay(TM) kit simultaneously detects both apoptosis associated cytotoxicity events as well as cell death due to necrosis. This simple and straightforward tool allows researchers to understand the overall health-status of their cell populations. In addition, this kit is useful to researchers investigating the effect of their novel drug or therapeutic as it allows them to assess their experimental outcome and evaluate the overall treatment of the cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry. Test Principle: Assessing potential cytotoxicity properties of chemical and biological agents is a mandatory requirement for the safe distribution of pharmaceuticals, vaccines, or additives associated with food product formulations. Necrosis vs Apoptosis BioAssay(TM) kit simultaneously detects both apoptosis associated cytotoxicity events as well as cell death due to necrosis. Apoptotic cells are identified using FLICA reagent probe. The FAM-FLICA probe covalently binds to active caspase enzymes, which are up-regulated during apoptosis, thus clearly labeling apoptotic cells for subsequent analysis. Non-apoptotic cells will not contain the active caspase enzymes required for FAM-FLICA to remain covalently bound within the cell structure. Loss of the integrity of the cell membrane, indicative of necrosis or late stage apoptosis, is detected using the vital staining dye, 7-aminoactinomycin D (7-AAD), a red fluorescing live/dead stain. This dye easily penetrates cell membrane-compromised cells, binding tightly to GC rich regions of the DNA. Because 7-AAD alone may not detect cells in the early stages of apoptosis, it is essential to use it in combination with the green-fluorescent FAM-FLICA apoptosis detection reagent. Combining these two different types of fluorescent cell-status-indicator reagents within a single test can reveal a significant percentage of cells that are 7-AAD-negative (membrane intact live cells) and yet FAM-FLICA positive (apoptotic). Kit Components: 50-100 tests: FAM-FLICA Poly Caspase Reagent (FAM-VAD-FMK), 2 vials 7-Aminoactinomycin D (7-AAD) vital dye, 1x0.26mg vial 10X Apoptosis Wash Buffer, 1x60mL bottle Fixative, 1x6mL 100-200 tests: FAM-FLICA Poly Caspase Reagent (FAM-VAD-FMK), 4 vials 7-Aminoactinomycin D (7-AAD) vital dye, 2x0.26mg vials 10X Apoptosis Wash Buffer, 2x60mL bottles Fixative, 1x6mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.