Source: Mitochondrial Antigen from bovine heart muscle Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Molecular Weight: Major Reactive Bands: 74kD (PDC E2) 41kD (PDH E1) 36kD (PDH E1) Minor Reactive Bands: 56kD (PDC E3 Protein X) 55kD (PDC E3 Lipoamide Dehydrogenase) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
90% (SDS-PAGE)
Form:
Supplied as a liquid in PBS, pH 7.4, 2.2mM EDTA, 0.08% lubrol, 20% glycerol.
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