IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. The Interleukin 2 (IL-2) BioAssay(TM) ELISA Kit (Human) is a solid phase immunoassay specially designed to measure Human IL-2 concentrations in in cell culture supernates, serum, plasma, and other biological fluids. Detection Range: 15.6-1000pg/ml Sensitivity: 0.1pg/ml Specificity: Recognizes natural and recombinant human IL-2, no detectable cross-reactivity with other relevant proteins. Assay Principle: The Interleukin 2 (IL-2) BioAssay(TM) ELISA Kit (Human) is a solid phase immunoassay specially designed to measure Human IL-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-2. It contains E. coli-expressed recombinant Human IL-2. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-2. The kit is analytically validated with ready to use reagents. Kit Components: Anti-Human IL-2 96-well strip microplate, Pre-coated, ready to use Standard, 2x10ng Human IL-2 Biotinylated antibody, 100X, 1x130ul Avidin-Biotin-Peroxidase Complex, 100X, 1x130ul Sample Diluent, 1x30ml Antibody Diluent, 1x12ml Avidin-Biotin-Peroxidase Diluent, 1x12ml Wash Buffer Concentrate, Powder for 1L Color Developing Reagent, 1x10ml Stop Solution, 1x10ml Storage and Stability: Store the unopened product at 2-8C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
